Article
[EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Jun 2012
Tao Xingkui, Zhang Aimin, Xue Jianping, Teng Jingtong, Lu Hedong, Xue Tao
Abstract excerpt
OBJECTIVE: To determine the optimal concentration and processing time of EMS mutation for suspension cells from Pinellia ternata. METHOD: Under four EMS concentration gradients (0.1% , 0.2%, 0.4%, 0.6%) and three processing time gradients (0.5, 1.0, 2.0 h), the suspension cells of P. ternata were mutagenized. RESULT AND CONCLUSION: The results showed that the survival rate was significantly different under the...
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