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Identification by Q-PCR of Trypanosoma cruzi lineage and determination of blood meal sources in triatomine gut samples in México.

Parasitology international - 1 Feb 2013

Ibáñez-Cervantes Gabriela, Martínez-Ibarra Alejandro, Nogueda-Torres Benjamín, López-Orduña Eduardo, Alonso Ana L, Perea Cynthia, Maldonado Teresa, Hernández José Manuel, León-Avila Gloria

Abstract excerpt

Triatomine vectors were collected on human dwellings in Michoacán México. Blood meal sources were identified by real time polymerase chain reaction (Q-PCR) using DNA extracted from triatomine guts. The assay was performed with one only specific primer set to amplify a fragment of the mitochondrial 12S ribosomal gene from vertebrate species. Also Trypanosoma cruzi parasites were detected in triatomine gut samples...

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PMID: 22995149
DOI: 10.1016/j.parint.2012.09.003

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