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Article

The widely used small subunit 18S rDNA molecule greatly underestimates true diversity in biodiversity surveys of the meiofauna.

Proceedings of the National Academy of Sciences of the United States of America - 2 Oct 2012

Tang Cuong Q, Leasi Francesca, Obertegger Ulrike, Kieneke Alexander, Barraclough Timothy G, Fontaneto Diego

Abstract excerpt

Molecular tools have revolutionized the exploration of biodiversity, especially in organisms for which traditional taxonomy is difficult, such as for microscopic animals (meiofauna). Environmental (eDNA) metabarcode surveys of DNA extracted from sediment samples are increasingly popular for surveying biodiversity. Most eDNA surveys use the nuclear gene-encoding small-subunit rDNA gene (18S) as a marker; however,...

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PMID: 22988084
DOI: 10.1073/pnas.1209160109
PMCID: PMC3479535

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