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Article

Establishment of outgrowth endothelial cells from peripheral blood.

Nature protocols - 1 Sept 2012

Martin-Ramirez Javier, Hofman Menno, van den Biggelaar Maartje, Hebbel Robert P, Voorberg Jan

Abstract excerpt

Blood outgrowth endothelial cells (BOECs) are important tools when investigating diagnostic and therapeutic approaches for vascular disease. In this protocol, mononuclear cells are isolated from peripheral blood and plated on type I collagen at ∼135,000 cells per cm(2) in endothelial cell differentiation medium. On average, 0.34 colonies of endothelial cells per milliliter of blood can be obtained. Colonies of...

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Identifiers

PMID: 22918388
DOI: 10.1038/nprot.2012.093

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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