Article
Characterization of stress and methylglyoxal inducible triose phosphate isomerase (OscTPI) from rice.
Plant signaling & behavior - 1 Oct 2012
Sharma Shweta, Mustafiz Ananda, Singla-Pareek Sneh L, Shankar Srivastava Prem, Sopory Sudhir Kumar
Abstract excerpt
As compared with plant system, triose phosphate isomerase (TPI), a crucial enzyme of glycolysis, has been well studied in animals. In order to characterize TPI in plants, a full-length cDNA encoding OscTPI was cloned from rice and expressed in E. coli. The recombinant OscTPI was purified to homogeneity and it showed Km value of 0.1281 ± 0.025 µM, and the Vmax value of 138.7 ± 16 µmol min (-1) mg (-1) which is...
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