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Detection of Streptococcus equi subspecies equi using a triplex qPCR assay

10 Aug 2012

Abstract excerpt

Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2h of sample...

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PMID: 22884566
DOI: 10.1016/j.tvjl.2012.07.007

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