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A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection

10 Jul 2012

Abstract excerpt

The polymerase chain reaction (PCR) is widely used as a diagnostic tool in clinical laboratories and is particularly effective for detecting and identifying infectious agents for which routine culture and microscopy methods are inadequate. Invasive fungal disease (IFD) is a major cause of morbidity and mortality in immunosuppressed patients, and optimal diagnostic criteria are contentious. Although PCR-based...

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PMID: 22808087
DOI: 10.1371/journal.pone.0040022

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