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Article

Development of a primer system to study abundance and diversity of the gene coding for alanine aminopeptidase pepN gene in Gram-negative soil bacteria.

Journal of microbiological methods - 1 Oct 2012

Enowashu Esther, Kandeler Ellen, Schloter Michael, Rasche Frank, Engel Marion

Abstract excerpt

A new set of primers was developed allowing the specific detection of the pepN gene (coding for alanine aminopeptidase) from Gram-negative bacteria. The primers were designed in silico by sequence alignments based on available DNA sequence data. The PCR assay was validated using DNA from selected pure cultures. The analysis of gene libraries from extracted DNA from different soil samples revealed a high diversity...

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PMID: 22796679
DOI: 10.1016/j.mimet.2012.07.001

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