Article
Ethanol-resistant mutants of Nicotiana plumbaginifolia are deficient in the expression of pollen and seed alcohol dehydrogenase activity.
Molecular & general genetics : MGG - 1 Jul 1990
Rousselin P, Lepingle A, Faure J D, Bitoun R, Caboche M
Abstract excerpt
Six independent mutant lines of Nicotiana plumbaginifolia resistant to ethanol, designated E3, E8, E101, E112, E144 and E251, were isolated as germinating seedlings on selective medium. In all cases, resistance to ethanol was conferred by a single recessive nuclear mutation at the same locus. Mutant seeds and pollen lacked detectable ADH activity, with the exception of E251 where a residual activity was detected....
Topics
- Alcohol Dehydrogenase
- Blotting, Northern
- Blotting, Western
- Crosses, Genetic
- Drug Resistance
- Enzyme Induction
- Ethanol
- Genes, Recessive
- Mutation
- Plants, Toxic
- Pollen