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Development of a real-time quadruplex PCR assay for simultaneous detection of nuc, Panton-Valentine leucocidin (PVL), mecA and homologue mecALGA251

11 Jun 2012

Abstract excerpt

BACKGROUND: The recent discovery of a mecA homologue (mecA(LGA251)) with a high level of variability between the two gene variants suggested that Staphylococcus aureus harbouring mecA(LGA251) could be wrongly identified as methicillin-susceptible S. aureus (MSSA), in the absence of antimicrobial susceptibility testing. METHODS: In this context we designed a real-time quadruplex PCR assay to distinguish...

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PMID: 22687894
DOI: 10.1093/jac/dks221

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