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Molecular detection of Leishmania isolated from Cutaneous leishmaniasis patients in Jask County, Hormozgan Province, Southern Iran, 2008

27 May 2012

Abstract excerpt

OBJECTIVE: To investigate on patients leishmanial infections in Jask County. METHODS: Impression smears were prepared from patients in 2008, all, were chequed for leishmanial infection by microscopy and molecular assays. Whole DNA was extracted using Proteinase K and Phenol/Chloroform/Isoamyl alcohol method. The variable segment on minicircles of kinetoplast DNA was amplified via a Nested-PCR technique using...

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PMID: 22647811
DOI: 10.1016/s1995-7645(12)60090-x

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