Article
Detection of disease resistance and susceptibility alleles in pigs using oligonucleotide microarray hybridization.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 May 2012
Pastoret Soumya, Ameels Hélène, Bossiroy Frédérique, Decreux Annabelle, De Longueville Françoise, Thomas Anne, Desmecht Daniel
Abstract excerpt
A multiplex DNA microarray chip aimed at the identification of allelic polymorphisms was developed for simultaneous detection of swine disease resistance genes underlying malignant hyperthermia (RYR), postweaning diarrhea, edema disease (FUT1), neonatal diarrhea (MUC4), and influenza (MX1). The on-chip detection was performed with fragmented polymerase chain reaction (PCR)-amplified products. Particular emphasis...
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