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Article

Sustained high-throughput polymerase chain reaction diagnostics during the European epidemic of <i>Bluetongue virus</i> serotype 8

23 Apr 2012

Abstract excerpt

A real-time reverse transcription polymerase chain reaction assay (PCR test) based on genome segment 10 of Bluetongue virus (BTV) was developed. The PCR test consists of robotized viral RNA isolation from blood samples and an all-in-one method including initial denaturation of genomic double-stranded RNA, reverse transcription polymerase chain reaction (RT-PCR), and real-time detection and analysis. Reference...

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PMID: 22529113
DOI: 10.1177/1040638712440986

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