Article
High-throughput microsatellite marker development in two sparid species and verification of their transferability in the family Sparidae.
Molecular ecology resources - 1 Jul 2012
Reid Kerry, Hoareau Thierry B, Bloomer Paulette
Abstract excerpt
Recently, 454 sequencing has emerged as a popular method for isolating microsatellites owing to cost-effectiveness and time saving. In this study, repeat-enriched libraries from two southern African endemic sparids (Pachymetopon blochii and Lithognathus lithognathus) were 454 GS-FLX sequenced. From these, 7370 sequences containing repeats (SCRs) were identified. A brief survey of 23 studies showed a significant...
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