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Article

New real-time PCR assay using allelic discrimination for detection and differentiation of equine herpesvirus-1 strains with A2254 and G2254 polymorphisms.

Journal of clinical microbiology - 1 Jun 2012

Smith Kathryn L, Li Yanqiu, Breheny Patrick, Cook R Frank, Henney Pamela J, Sells Stephen, Pronost Stéphane, Lu Zhengchun, Crossley Beate M, Timoney Peter J, Balasuriya Udeni B R

Abstract excerpt

A single-nucleotide polymorphism (A(2254) or G(2254)) in open reading frame 30 (ORF30) has been linked to the neuropathogenic phenotype of equine herpesvirus-1 (EHV-1). Identification of this polymorphism led to the development of a real-time PCR (rPCR) assay using allelic discrimination (E(2)) to distinguish between potentially neuropathogenic and nonneuropathogenic EHV-1 strains (G. P. Allen, J. Vet. Diagn....

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Identifiers

PMID: 22493339
DOI: 10.1128/JCM.00135-12
PMCID: PMC3372139

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