Discrimination of hybrid classes using cross-species amplification of microsatellite loci: methodological challenges and solutions in Daphnia.

doi:10.1111/j.1755-0998.2012.03142.x

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Article

Discrimination of hybrid classes using cross-species amplification of microsatellite loci: methodological challenges and solutions in Daphnia.

Molecular ecology resources - 1 Jul 2012

Thielsch A, Völker E, Kraus R H S, Schwenk K

Abstract excerpt

Microsatellite markers are important tools in population, conservation and forensic studies and are frequently used for species delineation, the detection of hybridization and introgression. Therefore, marker sets that amplify variable DNA regions in two species are required; however, cross-species amplification is often difficult, as genotyping errors such as null alleles may occur. To estimate the level of...

Topics

Alleles
Animals
DNA Primers
DNA, Mitochondrial
Daphnia
Genetic Markers
Genetic Variation
Genetics, Population
Genotype
Microsatellite Repeats

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Open in PubMed

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PMID: 22487563
DOI: 10.1111/j.1755-0998.2012.03142.x

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