Article
A novel duplex real-time PCR for HPIV-4 detects co-circulation of both viral subtypes among ill children during 2008.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 May 2012
Wang C Y T, Arden K E, Greer R, Sloots T P, Mackay I M
Abstract excerpt
The two subtypes of the human parainfluenzavirus type 4 (HPIV-4) are rarely sought in testing for acute respiratory illness (ARI) and this may be confounding our understanding of its role. This study presents a novel duplex real-time RT-PCR assay targeting the P gene that can detect and differentiate the two subtypes in a single reaction. Subtype-specific synthetic RNA positive controls were prepared and used to...
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