Article
A comparative transcriptomic study of an allotetraploid and its diploid progenitors illustrates the unique advantages and challenges of RNA-seq in plant species.
American journal of botany - 1 Feb 2012
Ilut Daniel C, Coate Jeremy E, Luciano Amelia K, Owens Thomas G, May Gregory D, Farmer Andrew, Doyle Jeff J
Abstract excerpt
PREMISE OF THE STUDY: RNA-seq analysis of plant transcriptomes poses unique challenges due to the highly duplicated nature of plant genomes. We address these challenges in the context of recently formed polyploid species and detail an RNA-seq experiment comparing the leaf transcriptome profile of an allopolyploid relative of soybean with the diploid species that contributed its homoeologous genomes. METHODS:...
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