Article
Multiplex PCR-based Alu insertion polymorphisms genotyping for identifying individuals of Japanese ethnicity.
Genomics - 1 Apr 2012
Asari Masaru, Omura Tomohiro, Oka Kumiko, Maseda Chikatoshi, Tasaki Yoshikazu, Shiono Hiroshi, Matsubara Kazuo, Matsuda Mitsuyoshi, Shimizu Keiko
Abstract excerpt
Discrimination of Alu insertions is a useful tool for geographic ancestry analysis, and is usually performed by Alu element amplification and agarose gel electrophoresis. Here, we have developed a new fluorescence-based method for multiple Alu genotyping in forensic identification. Allele frequencies were determined in 70 Japanese individuals, and we selected 30 polymorphic Alu insertions. Three primers were...
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