Article
SNaPshot® minisequencing analysis of multiple ancestry-informative Y-SNPs using capillary electrophoresis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012
Geppert Maria, Roewer Lutz
Abstract excerpt
This protocol describes a strategy for analyzing phylogenetic Y-SNPs in a hierarchical multiplex assay by utilizing the SNaPshot(®) Multiplex System. Step by step, the protocol assists in the appropriate selection of SNPs, the primer design, the set up of PCR/SBE reactions as well as in the analysis of the results. Furthermore, a forensic approach is highlighted, in which the most probable ancestry of an unknown...
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