Article
Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs.
Genetics and molecular research : GMR - 9 Nov 2011
Britto F B, Mendes D S F, Ogawa M, Cintra I H A, Diniz F M
Abstract excerpt
We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of each locus. A total of 78 loci were amplified in 76 samples; high polymorphism rates were...
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