Article
Development and evaluation of new primers for PCR-based identification of type II fimA of Porphyromonas gingivalis.
FEMS immunology and medical microbiology - 1 Apr 2012
Moon Ji-Hoi, Shin Seung-Il, Chung Jong-Hyuk, Lee Seung-Woo, Amano Atsuo, Lee Jin-Yong
Abstract excerpt
For more accurate PCR-based identification of Porphyromonas gingivalis harboring genotype II fimA, the most prevalent type in periodontitis patients, a new primer set was developed and evaluated. The previous type II primers hybridized to the DNA of P gingivalis strains harboring type Ib as well as type II fimA, while the new primers specifically amplified only the DNA fragment of type II fimA. In the...
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