Article
Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic.
Acta tropica - 1 Feb 2012
Fujita Megumi, Kato Hirotomo, Cáceres Abraham G, Gomez Eduardo A, Velez Lenin, Mimori Tatsuyuki, Zhang Feifei, Iwata Hiroyuki, Korenaga Masataka, Sakurai Tatsuya, Katakura Ken, Hashiguchi Yoshihisa
Abstract excerpt
Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI...
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