Article
[Isolation of microsatellite markers for Lateolabrax japonicus and polymorphic analysis].
Dong wu xue yan jiu = Zoological research - 1 Oct 2011
Zhao Yan, Ji Xiang-Shan, Zeng Yong-Qing, Ding Lei, Yang Ping-Ping, Wang Hui
Abstract excerpt
To investigate population structure and marker assisted breeding, fast isolation by AFLP of sequences containing repeats (FIASCO) and GenBank database mining were used to develop novel microsatellite markers for sea perch (Lateolabrax japonicus). Genomic DNA fragments containing SSR sequences were captured by hybridization to (GT)(13) biotin-labeled probe and were ligated to PMD18-T vector. Among 150 randomly...
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