Article
Plasmid typing and genetic context of AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: findings from a Spanish hospital 1999-2007.
The Journal of antimicrobial chemotherapy - 1 Jan 2012
Mata Caterina, Miró Elisenda, Alvarado Andrés, Garcillán-Barcia M Pilar, Toleman Mark, Walsh Timothy R, de la Cruz Fernando, Navarro Ferran
Abstract excerpt
OBJECTIVES: To gain insights into ampC transmission between bacterial strains. METHODS: We examined the genetic context of 117 acquired ampC genes from 27119 Enterobacteriaceae collected between 1999 and 2007. Plasmid analysis was carried out by PCR-based replicon or relaxase typing, S1-PFGE and Southern hybridization. I-CeuI/PFGE was used for isolates not characterized by plasmid analysis. PCR reactions were...
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