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Article

Somaclonal variation is induced de novo via the tissue culture process: a study quantifying mutated cells in Saintpaulia.

PloS one - 1 Jan 2011

Sato Mitsuru, Hosokawa Munetaka, Doi Motoaki

Abstract excerpt

BACKGROUND: The origin of somaclonal variation has not been questioned previously, i.e., "pre-existing mutations" in explants and "newly induced mutations" arising from the tissue culture process have not been distinguished. This is primarily because there has been no reliable molecular method for estimating or quantifying variation. METHODOLOGY/PRINCIPAL FINDINGS: We adopted a petal-variegated cultivar of...

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Identifiers

PMID: 21853148
DOI: 10.1371/journal.pone.0023541
PMCID: PMC3154937

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