Article
The Escherichia coli minB mutation resembles gyrB in defective nucleoid segregation and decreased negative supercoiling of plasmids.
Molecular & general genetics : MGG - 1 Mar 1990
Mulder E, El'Bouhali M, Pas E, Woldringh C L
Abstract excerpt
Nucleoid segregation in the Escherichia coli minB mutant and in cells that over-produce minB gene products appeared defective as measured from fluorescence micrographs. Electrophoretic resolution of topoisomers of plasmid isolates from the minB strain revealed a decreased level of negative supercoiling; in addition, multimerization was observed. Over-production of the minB gene product also resulted in a...
Topics
- Bacterial Proteins
- DNA, Bacterial
- DNA, Superhelical
- Escherichia coli
- Microscopy, Fluorescence
- Mutation
- Organelles
- Plasmids
