Article
[Genetic organization of transfer region of F-like plasmid pAP18-1].
Biulleten' eksperimental'noi biologii i meditsiny - 1 Sept 1990
Buianova N I, Shchipkov V P, Krivskaia K S, Pekhov A P
Abstract excerpt
The results of complementation analysis of nitrosoguanidine-induced mutants of F-like drd-plasmid pAP18-1 (Tc, ColV) testified to the existence of at least 3 tra regions (tra1, tra2, tra3) and regulating locus fin V in the genome of this plasmid. By means of molecular cloning of tra2 region and locus fin V of plasmid pAP18-1drd were located in Sall-fragment f5 (3.9 MD).
Topics
- Cloning, Molecular
- Conjugation, Genetic
- DNA Transposable Elements
- Electrophoresis
- Escherichia coli
- F Factor
- Genes, Bacterial
- Genetic Complementation Test
- Phenotype
- Restriction Mapping