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Article

Cloning and Characterization of an 11S Legumin, Car i 4, a Major Allergen in Pecan

30 Jun 2011

Abstract excerpt

Among tree nut allergens, pecan allergens remain to be identified and characterized. The objective was to demonstrate the IgE-binding ability of pecan 11S legumin and characterize its sequential IgE-binding epitopes. The 11S legumin gene was amplified from a pecan cDNA library and expressed as a fusion protein in Escherichia coli. The native 11S legumin in pecan extract was identified by mass spectrometry/mass...

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PMID: 21718052
DOI: 10.1021/jf2017447

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