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Article

Paralog-specific primers for the amplification of nuclear Loci in tetraploid barbels (barbus: cypriniformes).

The Journal of heredity - 1 Jan 2000

Gante Hugo F, Alves Maria Judite, Dowling Thomas E

Abstract excerpt

Thirty paralog-specific primers were developed, following an intron-primed exon-crossing strategy, for S7 and growth hormone genes in Barbus (subgenera Barbus and Luciobarbus). We found that paralog-specific amplification requires the use of only one paralog-specific primer, allowing their simultaneous use with universal exon-primed intron-crossing primers of broad taxonomic applicability. This hybrid annealing...

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Identifiers

PMID: 21705490
DOI: 10.1093/jhered/esr059

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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