Article
Tn1000-mediated insertion mutagenesis of the histidine utilization (hut) gene cluster from Klebsiella aerogenes: genetic analysis of hut and unusual target specificity of Tn1000.
Journal of bacteriology - 1 Oct 1990
Schwacha A, Cohen J A, Gehring K B, Bender R A
Abstract excerpt
The histidine utilization (hut) genes from Klebsiella aerogenes were cloned in both orientations into the HindIII site of plasmid pBR325, and the two resulting plasmids, pCB120 and pCB121, were subjected to mutagenesis with Tn1000. The insertion sites of Tn1000 into pCB121 were evenly distributed throughout the plasmid, but the insertion sites into pCB120 were not. There was a large excess of Tn1000 insertions in...
Topics
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Histidine
- Histidine Ammonia-Lyase
- Klebsiella pneumoniae
- Multigene Family
- Mutation