Article
A novel small protein of Bacillus subtilis involved in spore germination and spore coat assembly.
Bioscience, biotechnology, and biochemistry - 1 Jan 2011
Kodama Takeko, Matsubayashi Takeshi, Yanagihara Tadayoshi, Komoto Hiroyuki, Ara Katsutoshi, Ozaki Katsuya, Kuwana Ritsuko, Imamura Daisuke, Takamatsu Hiromu, Watabe Kazuhito, Sekiguchi Junichi
Abstract excerpt
Two small genes named sscA (previously yhzE) and orf-62, located in the prsA-yhaK intergenic region of the Bacillus subtilis genome, were transcribed by SigK and GerE in the mother cells during the later stages of sporulation. The SscA-FLAG fusion protein was produced from T(5) of sporulation and incorporated into mature spores. sscA mutant spores exhibited poor germination, and Tricine-SDS-PAGE analysis showed...
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