Article
Induction and flow cytometry identification of tetraploids from seed-derived explants through colchicine treatments in Catharanthus roseus (L.) G. Don.
Journal of biomedicine & biotechnology - 1 Jan 2011
Xing Shi-Hai, Guo Xin-Bo, Wang Quan, Pan Qi-Fang, Tian Yue-Sheng, Liu Pin, Zhao Jing-Ya, Wang Guo-Feng, Sun Xiao-Fen, Tang Ke-Xuan
Abstract excerpt
The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that...
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