Article
Comparative evaluation of combined-disk tests using different boronic acid compounds for detection of klebsiella pneumoniae carbapenemase-producing enterobacteriaceae clinical isolates.
Journal of clinical microbiology - 1 Aug 2011
Tsakris Athanassios, Themeli-Digalaki Katerina, Poulou Aggeliki, Vrioni Georgia, Voulgari Evangelia, Koumaki Vasiliki, Agodi Antonella, Pournaras Spyros, Sofianou Danai
Abstract excerpt
The accurate phenotypic detection of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae is an increasing necessity worldwide. We evaluated the performance of boronic acid combined-disk tests using as substrate imipenem or meropenem and as inhibitor of KPC production 300 μg aminophenylboronic acid (APBA), 600 μg APBA, or 400 μg phenylboronic acid (PBA). Tests were considered positive when an...
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