Article
Single strand conformation polymorphism is a sensitive method for screening nucleotide variations in Mycosphaerella graminicola.
Communications in agricultural and applied biological sciences - 1 Jan 2010
Siah A, Tisserant B, El Chartouni L, Deweer C, Roisin-Fichter C, Sanssené J, Durand R, Reignault Ph, Halama P
Abstract excerpt
Single Strand Conformation Polymorphism (SSCP) and sequencing were performed in order to assess molecular polymorphism of mating type sequences in the heterothallic ascomycete Mycosphaerella graminicola, the causal agent of Septoria tritici blotch of wheat. The screening was undertaken on mat1-1 and mat1-2 partial sequences of 341 and 657 bp, respectively, amplified with multiplex PCR from 510 French...
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