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<i>Wolbachia</i> detection: an assessment of standard PCR Protocols

18 Jan 2011

Abstract excerpt

Wolbachia is a large monophyletic genus of intracellular bacteria, traditionally detected using PCR assays. Its considerable phylogenetic diversity and impact on arthropods and nematodes make it urgent to assess the efficiency of these screening protocols. The sensitivity and range of commonly used PCR primers and of a new set of 16S primers were evaluated on a wide range of hosts and Wolbachia strains. We show...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 21481216
DOI: 10.1111/j.1755-0998.2010.02955.x

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