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Article

SnRK2.6/OST1 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis of K50N and D160A mutants.

Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Mar 2011

Yunta Cristina, Martinez-Ripoll Martin, Albert Armando

Abstract excerpt

The SnRK2.6 (SNF1-related kinase 2.6) gene from Arabidopsis thaliana encodes the serine/threonine protein kinase SnRK2.6/OST1 (OPEN STOMATA 1). It plays a central role in the drought-tolerance mechanism. OST1 is in fact the main positive effector in the hydric stress response. The SnRK2.6 gene was cloned into the pGEX4T1 plasmid, mutated and expressed in Escherichia coli, allowing purification to homogeneity in...

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Identifiers

PMID: 21393844
DOI: 10.1107/S1744309110053807
PMCID: PMC3053164

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