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Article

Construction of Pseudomonas syringae pv. tomato DC3000 mutant and polymutant strains.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Kvitko Brian H, Collmer Alan

Abstract excerpt

Redundancy between Pseudomonas syringae pv. tomato DC3000 virulence factors has made their characterization difficult. One method to circumvent redundancy for phenotypic characterization is to simultaneously delete all redundant factors through the generation of polymutant strains. Described here are methods by which single and polymutant strains of DC3000 can be generated through the use of the small mobilizable...

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PMID: 21359804
DOI: 10.1007/978-1-61737-998-7_10

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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