Article
A duplex real-time reverse transcription polymerase chain reaction for the detection and quantitation of avian leukosis virus subgroups A and B.
Journal of virological methods - 1 May 2011
Zhou Gang, Cai Wenbo, Liu Xiaolei, Niu Chengming, Gao Caixia, Si Changde, Zhang Wei, Qu Liandong, Han Lingxia
Abstract excerpt
Avian leukosis is a disease that is spreading widely in the world causing large economic losses to the poultry industry. In this study, a duplex quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assay was developed to detect and quantify avian leukosis virus subgroups A and B (ALVA/B). The assay was optimised to measure viral gp85 and chicken housekeeping (β-actin) genes. The result...
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