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Reevaluating and Optimizing Polyomavirus BK and JC Real-Time PCR Assays To Detect Rare Sequence Polymorphisms

17 Feb 2011

Abstract excerpt

PCR-based molecular assays have a central role in polyomavirus diagnostics. To assure optimal performance, target sequences should be regularly updated according to newly available sequences. The aim of this study was to review our in-house polyomavirus BK (BKV) and JC (JCV) real-time PCR assays. Database analysis revealed variations in the BKV target region which might affect the assay performance, while no...

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PMID: 21325560
DOI: 10.1128/jcm.02008-10

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