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A Sensitive Assay for Virus Discovery in Respiratory Clinical Samples

24 Jan 2011

Abstract excerpt

In 5-40% of respiratory infections in children, the diagnostics remain negative, suggesting that the patients might be infected with a yet unknown pathogen. Virus discovery cDNA-AFLP (VIDISCA) is a virus discovery method based on recognition of restriction enzyme cleavage sites, ligation of adaptors and subsequent amplification by PCR. However, direct discovery of unknown pathogens in nasopharyngeal swabs is...

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PMID: 21283679
DOI: 10.1371/journal.pone.0016118

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