Article
Application of new assays for rapid confirmation and genotyping of isolates of rubella virus.
Journal of medical virology - 1 Jan 2011
Feng Yan, Santibanez Sabine, Appleton Hazel, Lu Yiyu, Jin Li
Abstract excerpt
Rubella virus (RV) isolation is recommended by the WHO Measles and Rubella Labnet for studying the etiology and epidemiology of rubella. However, the absence of cytopathologic effects (CPE) in many of the cell lines used commonly makes it difficult to confirm RV growth. In this study, two assays amplifying RV cDNA were developed and validated in order to confirm and genotype RV isolates after cell culture. A SYBR...
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