Article
Cryopreservation of Drosophila melanogaster embryos.
Nature - 10 May 1990
Steponkus P L, Myers S P, Lynch D V, Gardner L, Bronshteyn V, Leibo S P, Rall W F, Pitt R E, Lin T T, MacIntyre R J
Abstract excerpt
There is an urgent need to preserve the ever-increasing number (greater than 30,000) of different genetic strains of D. melanogaster that are maintained in national and international stock centres and in the laboratories of individual investigators. In all cases, the stocks are maintained as adult populations and require transfer to fresh medium every two to four weeks. This is not only costly in terms of...
Topics
- Animals
- Cryopreservation
- Culture Techniques
- Desiccation
- Drosophila melanogaster
- Ethylene Glycol
- Ethylene Glycols
- Genotype
- Larva
- Nitrogen
- Permeability
- Polypropylenes