Article
Inhibition of Arabidopsis O-acetylserine(thiol)lyase A1 by tyrosine nitration.
The Journal of biological chemistry - 7 Jan 2011
Alvarez Consolación, Lozano-Juste Jorge, Romero Luís C, García Irene, Gotor Cecilia, León José
Abstract excerpt
The last step of sulfur assimilation is catalyzed by O-acetylserine(thiol)lyase (OASTL) enzymes. OASTLs are encoded by a multigene family in the model plant Arabidopsis thaliana. Cytosolic OASA1 enzyme is the main source of OASTL activity and thus crucial for cysteine homeostasis. We found that nitrating conditions after exposure to peroxynitrite strongly inhibited OASTL activity. Among OASTLs, OASA1 was markedly...
Topics
- Amino Acid Sequence
- Arabidopsis
- Binding Sites
- Biocatalysis
- Carbon-Oxygen Lyases
- Enzyme Inhibitors
- Models, Molecular
- Mutation
- Peroxynitrous Acid
- Plant Extracts
- Protein Conformation
- Protein Processing, Post-Translational
- Tyrosine