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Fertile plant regeneration from cryopreserved calli of Oryza rufipogon Griff. and assessment of variation in the progeny of regenerated plants.

Plant cell reports - 1 Dec 2010

Zeliang P K, Pattanayak A, Iangrai B, Khongwir E A, Sarma B K

Abstract excerpt

A protocol was developed for preservation of calli of Oryza rufipogon Griff. in liquid nitrogen (-196°C). Optimal conditions for cryoprotection, pre-freezing and post-thaw recovery of calli were identified. Cryoprotectant treatment at low temperature, pre-freezing in isopropanol bath for 1 h at -70°C, rapid thawing and proper removal of cryoprotectant were critical for post-thaw survival. Genetic fidelity of the...

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PMID: 20960202
DOI: 10.1007/s00299-010-0932-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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