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Interlaboratory Evaluation of Different Extraction and Real-Time PCR Methods for Detection ofCoxiella burnetiiDNA in Serum

9 Sept 2010

Abstract excerpt

In the Netherlands, there is an ongoing and unparalleled outbreak of Q fever. Rapid and reliable methods to identify patients infected with Coxiella burnetii, the causative agent of Q fever, are urgently needed. We evaluated the performance of different DNA extraction methods and real-time PCR assays that are in use in seven diagnostic or reference laboratories in the Netherlands. A low degree of variation in the...

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PMID: 20826645
DOI: 10.1128/jcm.01006-10

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