Article
Further diversity of the Staphylococcus intermedius group and heterogeneity in the MboI restriction site used for Staphylococcus pseudintermedius species identification.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Sept 2010
Slettemeås Jannice Schau, Mikalsen Jarle, Sunde Marianne
Abstract excerpt
Species identification of 200 beta-hemolysin-producing canine staphylococcal isolates was performed using a recently described polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method (based on MboI restriction of a pta gene fragment), supplemented with biochemical testing and sequencing of housekeeping genes. The PCR-RFLP method misclassified a small fraction (approximately 1%) of the...
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