Article
Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution.
BMB reports - 1 Aug 2010
Jin Xiao-Fen, Zhu Bo, Peng Ri-He, Jiang Hai-hua, Chen Jian-Min, Zhuang Jing, Zhang Jian, Yao Quan-Hong, Xiong Ai-Sheng
Abstract excerpt
In this study, we cloned the ERF-B3 subfamily transcription factor gene BnaERF-B3-hy15 from Brassica napus L. Huyou15. This 600 bp gene encodes a 199 amino acid classic ethylene responsive factor (ERF), which shown no binding or very weak binding GCC box-binding activity by the yeast one-hybrid assay. We used gene shuffling and the yeast one-hybrid system to obtain three mutated sequences that can bind to the GCC...
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