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[A nested multiplex PCR assay for rapid subtyping the prevailing HIV-1 strains in Guangxi].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Dec 2009

Su Qi-jian, Zhou Ping, Bi Zhi-you, Xiao Xin, Wu Shu-zhi, Cen Ping, Deng Wei, Shao Yi-ming, Liang Hao

Abstract excerpt

OBJECTIVE: To establish a rapid nested multiplex PCR assay for subtyping HIV-1 CRF01_AE, CRF07_BC, CRF08_BC, B, and C strains prevailing in Guangxi. METHOD: Subtype-specific primers were designed for these subtypes based on their gag sequences. The subtypes of HIV-1 samples from Guangxi were determined by nested multiplex PCR and DNA sequencing and phylogenetic analysis, respectively, and then the sensitivity and...

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PMID: 20718364

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