Detection of pathogenic elephant endotheliotropic herpesvirus in routine trunk washes from healthy adult Asian elephants (Elephas maximus) by use of a real-time quantitative polymerase chain reaction assay.

Abstract excerpt

OBJECTIVE: To investigate the pathogenesis and transmission of elephant endotheliotropic herpesvirus (EEHV1) by analyzing various elephant fluid samples with a novel EEHV1-specific real-time PCR assay. ANIMALS: 5 apparently healthy captive Asian elephants (Elephas maximus) from the same herd. PROCEDURES: A real-time PCR assay was developed that specifically detects EEHV1. The assay was used to evaluate paired...

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